Title: Tank Stabilization


Background:(Daniza March 1, 2011)


  • Purpose:

That the trout can survive with enough oxygen, the right care, and management of their environment.
  • Hypothesis:

If we use The cycling & water conditioning kit correctly, then the number of trout deaths will decrease, because it contains NIte-Out 2 II, which will help establish, promote/stabilize, and maintain nitrification in aquarium water, and it will eliminate the toxic effects of ammonia.
  • Variables:

-Independent : The Temperature of the water in the tank, pH, NH3, Chlorine.
-Dependent : The number of trout deaths.
-Control : The kits that are being used.
-Control Group : Not needed for this experiment.

Materials(Catherine 2/19/11):

  • 50 Gallon tank
  • 50 Gallons of H2O
  • JBJ Aquarium Chillers
  • Red Sea pH/Alkalinity Mini-Lab Test Kit
  • API Ammonia (NH3/ NH4+) Test Kit
  • DO and CL testing kit
  • Micro-Lift Aquarium Cycling
  • Water conditioning kit
  • Thermometer
  • Fish food
  • UV Aquarium Sterilizers


Procedures (Catherine 3/1/11):

We checked:
The temperature- everday.
The tank's pH- once a week:
  1. Use the low pH or high pH indicator and color scale as appropiate.
  2. Clean a test tube by rinsing it with water to be tested.
  3. Fill the test tube to the 6 mL mark with water to be tested.
  4. Add 3 drops of pH indicator, close the test tube with the stopper and shake gently for 10 seconds.
  5. Compare the color with the color scale, by holding the test tube in a verticle position, and read off the pH value of the color most similar to the sample.
The ammonia levels- once a week:
  1. Fill a clean test tube with 5 mL of water to be tested.
  2. Add 8 drops from Ammonia Test Solution Bottle 2, holding the dropper bottle upside down in a completely vertical position to assure uniform drops.
  3. Cap the tube and shake vigorously for 5 seconds.
  4. Wait for 5 minutes for the color to develop.
  5. Read the test results by comparing the color of the solution to the appropiate Ammonia Color Card.
The dissolved oxygen levels- once a month:
The chlorine levels- once a month:
  1. We filled a test tube to the 5mL line with the sample water.
  2. Add one *Chlorine DPD #1R Tablet (6999). Cap tube and mix until table disintergrates.
  3. Insert test tube into the top of the Octo-Slide Viewer. Slide the Octa-Slide Bar (3405) into the viewer. Match sample color to a color standard.
  4. Record as ppm Free Available Chlorine.
We also kept the water temperature between 50-60 degrees Fahrenheit and make sure they were given the right amount of food.

Result

  • Table(Kail 2011/03/03)


Date
Death
2010-11-08
1
2010-11-09
4
2010-11-10
3
2010-11-12
4
2010-11-15
4
2010-11-16
2
2010-11-17
3
2010-11-22
3
2010-11-30
0
2010-12-01
0
2010-12-02
0
2010-12-06
0
2010-12-07
0
2010-12-08
1
2010-12-09
0
2010-12-10
0
2010-12-13
0
2010-12-14
0
2010-12-15
0
2010-12-16
0
2010-12-17
0
2010-12-20
0
2010-12-21
0
2010-12-22
0
2011-01-03
0
2011-01-04
0
2011-01-05
0
2011-01-06
0
2011-01-07
0
2011-01-10
0
2011-01-11
0
2011-01-13
0
2011-01-14
0
2011-01-15~2011-01-28
0
2011-01-31
1
2011-02-01~2011-02-24
0
2011-02-25
1
2011-02-28~2011-03-03
0

GRAPH (Jordan 3/3/2011)



Conclusion

(Rich 1/4/11)
Our hypothesis was correct, the NIte-Out 2 II sucessfully destroyed the lethal effects of ammonia and our trout are alive and perfectly healthy.We can improve our lab by taking our tests more frequently and making sure we put up all of the data that we have collected the day we take it instead of waiting and possibility losing all of the data that we had collected.